Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the global COVID-19 pandemic and the lack of therapeutics hinders pandemic control^1-2. Although lung disease is the primary clinical outcome in COVID-19 patients^1-3, how SARS-CoV-2 induces tissue pathology in the lung remains elusive. Here we describe a high-throughput platform to generate tens of thousands of self-organizing, nearly identical, and genetically matched human lung buds derived from human pluripotent stem cells (hPSCs) cultured on micropatterned substrates. Strikingly, in vitro-derived human lung buds resemble fetal human lung tissue and display in vivo-like proximo-distal coordination of alveolar and airway tissue differentiation whose 3D epithelial self

SUBMITTER: Rosado-Olivieri EA 

PROVIDER: S-EPMC7805464 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature