Molecular asymmetry in the cephalochordate embryo revealed by single-blastomere transcriptome profiling.

ABSTRACT: Studies in various animals have shown that asymmetrically localized maternal transcripts play important roles in axial patterning and cell fate specification in early embryos. However, comprehensive analyses of the maternal transcriptomes with spatial information are scarce and limited to a handful of model organisms. In cephalochordates (amphioxus), an early branching chordate group, maternal transcripts of germline determinants form a compact granule that is inherited by a single blastomere during cleavage stages. Further blastomere separation experiments suggest that other transcripts associated with the granule are likely responsible for organizing the posterior structure in amphioxus; however, the identities of these determinants remain unknown. In this study, we used high-throughput

SUBMITTER: Lin CY 

PROVIDER: S-EPMC7806126 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature