Cytosine methylation of mitochondrial DNA at CpG sequences impacts transcription factor A DNA binding and transcription.

ABSTRACT: In eukaryotes, cytosine methylation of nuclear DNA at CpG sequences (^5mCpG) regulates epigenetic inheritance through alterations in chromatin structure. However, mitochondria lack nucleosomal chromatin, therefore the molecular mechanisms by which ^5mCpG influences mitochondria must be different and are as yet unknown. Mitochondrial Transcription Factor A (TFAM) is both the primary DNA-compacting protein in the mitochondrial DNA (mtDNA) nucleoid and a transcription-initiation factor. TFAM must encounter hundreds of CpGs in mtDNA, so the occurrence of ^5mCpG has the potential to impact TFAM-DNA recognition. We used biophysical approaches to determine whether ^5mCpG alters any TFAM-dependent activities. ^5mCpG in the heavy strand promoter (HS

SUBMITTER: Dostal V 

PROVIDER: S-EPMC7806247 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature