Immunohistochemical Analysis of Histone H3 Modification in Newt Tail Tissue Cells following Amputation.

ABSTRACT:

Background

Newts have impressive regenerative capabilities, but it remains unclear about the role of epigenetic regulation in regeneration process. We herein investigated histone modifications in newt tail tissue cells following amputation.

Methods and results

Iberian ribbed male newts (6-8 months old) were suffered to about 1.5 cm length of amputation of their tails for initiating regeneration process, and the residual stump of tail tissues was collected for immunohistochemical analysis 3 days later. Compared to the tissue cells of intact tails, c-kit-positive stem cells and PCNA-positive proliferating cells were significantly higher in tails suffered to amputation (P < 0.001). Amputation also significantly induced the acetylation of H3K9, H3K14, and H3K27 in cells o

SUBMITTER: Wu JW 

PROVIDER: S-EPMC7806392 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature