Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE.

ABSTRACT: Human pluripotent stem cell-derived retinal pigment epithelium (RPE) transplantation is currently under evaluation as treatment for macular degeneration. For therapeutic applications, cryostorage during cell production is typically needed with potential consequences to cell functionality. We have previously shown that the culture substrate affects human embryonic stem cell-derived RPE (hESC-RPE) properties in fresh cultures. Here, we aimed to further identify the role of RPE basement membrane proteins type IV collagen (Col-IV), laminin (LN), and nidogen-1 in the maturation and functionality of hESC-RPE after cryopreservation. In addition to cell attachment and morphology, transepithelial electrical resistance, expression of key RPE proteins, phagocytosis capacity and Ca²⁺ signal

SUBMITTER: Viheriala T 

PROVIDER: S-EPMC7806758 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature