Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction.

ABSTRACT:

Background & aims

YAP (Yap1) and TAZ (Wwtr1) are transcriptional co-activators and downstream effectors of the Hippo pathway, which play crucial roles in organ size control and cancer pathogenesis. Genetic deletion of YAP/TAZ has shown their critical importance for embryonic development of the heart, vasculature, and gastrointestinal mesenchyme. The aim of this study was to determine the functional role of YAP/TAZ in adult smooth muscle cells in vivo.

Methods

Because YAP and TAZ are mutually redundant, we used YAP/TAZ double-floxed mice crossed with mice that express tamoxifen-inducible CreER^T2 recombinase driven by the smooth muscle-specific myosin heavy chain promoter.

Results

Double-knockout of YAP/TAZ in adult smooth muscle causes lethality within 2 wee

SUBMITTER: Daoud F 

PROVIDER: S-EPMC7806867 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature