TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages.

ABSTRACT: Identifying endogenous tissue stem cells remains a key challenge in developmental and regenerative biology. To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers with ultrastructural morphology is highly beneficial. Here, we realise this through workflows of multi-resolution immuno-correlative light and electron microscopy (iCLEM) methodologies. Taking advantage of the antigenicity preservation of the Tokuyasu technique, we have established robust protocols and workflows and provide a side-by-side comparison of iCLEM used in combination with scanning EM (SEM), scanning TEM (STEM), or transmission EM (TEM). Evaluation of the applications and advantages of each method highlights their practicality for th

SUBMITTER: Oorschot V 

PROVIDER: S-EPMC7806999 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature