Multi-pronged approach to human mesenchymal stromal cells senescence quantification with a focus on label-free methods.

ABSTRACT: Human mesenchymal stromal cells (hMSCs) have demonstrated, in various preclinical settings, consistent ability in promoting tissue healing and improving outcomes in animal disease models. However, translation from the preclinical model into clinical practice has proven to be considerably more difficult. One key challenge being the inability to perform in situ assessment of the hMSCs in continuous culture, where the accumulation of the senescent cells impairs the culture's viability, differentiation potential and ultimately leads to reduced therapeutic efficacies. Histochemical [Formula: see text]-galactosidase staining is the current standard for measuring hMSC senescence, but this method is destructive and not label-free. In this study, we have investigated alternatives in quantification

SUBMITTER: Zhai W 

PROVIDER: S-EPMC7807049 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature