NMR Chemical Exchange Measurements Reveal That N⁶-Methyladenosine Slows RNA Annealing.

ABSTRACT: N⁶-Methyladenosine (m⁶A) is an abundant epitranscriptomic modification that plays important roles in many aspects of RNA metabolism. While m⁶A is thought to mainly function by recruiting reader proteins to specific RNA sites, the modification can also reshape RNA-protein and RNA-RNA interactions by altering RNA structure mainly by destabilizing base pairing. Little is known about how m⁶A and other epitranscriptomic modifications might affect the kinetic rates of RNA folding and other conformational transitions that are also important for cellular activity. Here, we used NMR R_1ρ relaxation dispersion and chemical exchange saturation transfer to noninvasively and site-specifically measure nucleic acid hybridization kinetics

SUBMITTER: Shi H 

PROVIDER: S-EPMC7807175 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature