Occurrence and expression of genes encoding methyl-compound production in rumen bacteria.

ABSTRACT:

Background

Digestive processes in the rumen lead to the release of methyl-compounds, mainly methanol and methylamines, which are used by methyltrophic methanogens to form methane, an important agricultural greenhouse gas. Methylamines are produced from plant phosphatidylcholine degradation, by choline trimethylamine lyase, while methanol comes from demethoxylation of dietary pectins via pectin methylesterase activity. We have screened rumen metagenomic and metatranscriptomic datasets, metagenome assembled genomes, and the Hungate1000 genomes to identify organisms capable of producing methyl-compounds. We also describe the enrichment of pectin-degrading and methane-forming microbes from sheep rumen contents and the analysis of their genomes via metagenomic assembly.

Results

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SUBMITTER: Kelly WJ 

PROVIDER: S-EPMC7807696 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature