High-resolution analysis of cell-state transitions in yeast suggests widespread transcriptional tuning by alternative starts.

ABSTRACT:

Background

The start and end sites of messenger RNAs (TSSs and TESs) are highly regulated, often in a cell-type-specific manner. Yet the contribution of transcript diversity in regulating gene expression remains largely elusive. We perform an integrative analysis of multiple highly synchronized cell-fate transitions and quantitative genomic techniques in Saccharomyces cerevisiae to identify regulatory functions associated with transcribing alternative isoforms.

Results

Cell-fate transitions feature widespread elevated expression of alternative TSS and, to a lesser degree, TES usage. These dynamically regulated alternative TSSs are located mostly upstream of canonical TSSs, but also within gene bodies possibly encoding for protein isoforms. Increased upstream alternative TSS

SUBMITTER: Chia M 

PROVIDER: S-EPMC7807719 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature