Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)2(dppx)2+ toward Silicon Nanodots.
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ABSTRACT: A ratiometric DNA sensor was developed based on fluorescent silicon nanodots (SiNDs) and Ru(bpy)2(dppx)2+. The absorption spectrum of Ru(bpy)2(dppx)2+ has significant overlap with both the excitation and emission spectra of SiNDs. Therefore, fluorescence quenching of Ru(bpy)2(dppx)2+ toward SiNDs can occur on account of the strong inner filter effect. The effect of quenching is not influenced by the specific binding between Ru(bpy)2(dppx)2+ and DNA. Fluorescence turn-on detection of DNA can be performed employing Ru(bpy)2(dppx)2+ and SiNDs as the response and reference signals, respectively. Using SiND-Ru(bpy)2(dppx)2+, a convenient, sensitive, rapid, and prec
SUBMITTER: Zhang Y
PROVIDER: S-EPMC7808131 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
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