Metabolic Glycan Labeling-Based Screen to Identify Bacterial Glycosylation Genes.

ABSTRACT: Bacterial cell surface glycans are quintessential drug targets due to their critical role in colonization of the host, pathogen survival, and immune evasion. The dense cell envelope glycocalyx contains distinctive monosaccharides that are stitched together into higher order glycans to yield exclusively bacterial structures that are critical for strain fitness and pathogenesis. However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging. Bacteria produce glycans containing rare sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the identification of their biosynthesis machinery. To ease the study of bacterial glycans in the absence of detailed structural information, we used metabolic glycan labeling to d

SUBMITTER: Moulton KD 

PROVIDER: S-EPMC7808405 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature