The identification of novel promoters and terminators for protein expression and metabolic engineering applications in Kluyveromyces marxianus.

ABSTRACT: The K. marxianus has emerged as a potential yeast strain for various biotechnological applications. However, the limited number of available genetic tools has hindered the widespread usage of this yeast. In the current study we have expanded the molecular tool box by identifying novel sets of promoters and terminators for increased recombinant protein expression in K. marxianus. The previously available transcriptomic data were analyzed to identify top 10 promoters of highest gene expression activity. We further characterized and compared strength of these identified promoters using eGFP as a reporter protein, at different temperatures and carbon sources. To examine the regulatory region driving protein expression, serially truncated shorter versions of two selected strong pr

SUBMITTER: Kumar P 

PROVIDER: S-EPMC7808952 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature