In vivo tracking of ¹⁴C thymidine labeled mesenchymal stem cells using ultra-sensitive accelerator mass spectrometry.

ABSTRACT: Despite the tremendous advancements made in cell tracking, in vivo imaging and volumetric analysis, it remains difficult to accurately quantify the number of infused cells following stem cell therapy, especially at the single cell level, mainly due to the sensitivity of cells. In this study, we demonstrate the utility of both liquid scintillator counter (LSC) and accelerator mass spectrometry (AMS) in investigating the distribution and quantification of radioisotope labeled adipocyte derived mesenchymal stem cells (AD-MSCs) at the single cell level after intravenous (IV) transplantation. We first show the incorporation of ¹⁴C-thymidine (5 nCi/ml, 24.2 ng/ml) into AD-MSCs without affecting key biological characteristics. These cells were then utilized to track and quantify the di

SUBMITTER: Oh MS 

PROVIDER: S-EPMC7809063 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature