Implication of therapeutic outcomes associated with molecular characterization of paediatric aplastic anaemia.

ABSTRACT:

Objectives

Severe aplastic anemia is characterized by a hypocellular bone marrow and peripheral cytopenia. Mesenchymal stem cells (MSCs) play a crucial role in haematopoietic stem cells (HSCs) development and the development of microenvironment suitable for hematopoiesis. Molecular characterization of telomere maintenance pathway and gene expression profiling of MSCs can be important for the therapeutic interventions among paediatric aplastic anaemia patients.

Methods

The study involved paediatric aplastic anaemia patients (n = 10) and age matched paediatric healthy donors (n = 8). Peripheral blood samples were collected from the individuals. Average leucocyte telomere length and gene expression of the telomere maintenance genes were determined by quantitative real time PCR.

SUBMITTER: Adhikari S 

PROVIDER: S-EPMC7809188 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature