Human Keratinocytes Adopt Neuronal Fates After In Utero Transplantation in the Developing Rat Brain.

ABSTRACT: Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of transcription factors. In this article we tested the hypothesis that human keratinocytes can adopt neural fates after culturing them in suspension with a neural medium. Initially, keratinocytes expressed Keratins and Vimentin. After neural induction, transcriptional upregulation of NESTIN, SOX2, VIMENTIN, SOX1, and MUSASHI1 was observed, concomitant with significant increases in NESTIN detected by immunostaining. However, in vitro differentiation did not y

SUBMITTER: Tenorio-Mina A 

PROVIDER: S-EPMC7809298 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature