PacBio full-length transcriptome of wild apple (Malus sieversii) provides insights into canker disease dynamic response.

ABSTRACT:

Background

Valsa canker is a serious disease in the stem of Malus sieversii, caused by Valsa mali. However, little is known about the global response mechanism in M. sieversii to V. mali infection.

Results

Phytohormone jasmonic acid (JA) and salicylic acid (SA) profiles and transcriptome analysis were used to elaborate on the dynamic response mechanism. We determined that the JA was initially produced to respond to the necrotrophic pathogen V. mali infection at the early response stage, then get synergistically transduced with SA to respond at the late response stage. Furthermore, we adopted Pacific Biosciences (PacBio) full-length sequencing to identify differentially expressed transcripts (DETs) during the canker response stage. We obtained 52,538 full-length transcripts,

SUBMITTER: Liu X 

PROVIDER: S-EPMC7809858 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature