Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium.

ABSTRACT: One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the lack of a consensus method for EV separation. This may also explain the diversity of the experimental results, as co-separated soluble proteins and lipoproteins may impede the interpretation of experimental findings. In this study, we comprehensively evaluated the EV yields and sample purities of three most popular EV separation methods, ultracentrifugation, precipitation and size exclusion chromatography combined with ultrafiltration, along with a microfluidic tangential flow filtration device, Exodisc, in three commonly used biological samples, cell culture medium, human urine and plasma. Single EV phenotyping and density-gradient ultracentrifugation were used to understand the proportion o

SUBMITTER: Dong L 

PROVIDER: S-EPMC7810129 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature