Validation of multiplex PCR sequencing assay of SIV.

ABSTRACT:

Background

The generation of accurate and reproducible viral sequence data is necessary to understand the diversity present in populations of RNA viruses isolated from clinical samples. While various sequencing methods are available, they often require high quality templates and high viral titer to ensure reliable data.

Methods

We modified a multiplex PCR and sequencing approach to characterize populations of simian immunodeficiency virus (SIV) isolated from nonhuman primates. We chose this approach with the aim of reducing the number of required input templates while maintaining fidelity and sensitivity. We conducted replicate sequencing experiments using different numbers of quantified viral RNA (vRNA) or viral cDNA as input material. We performed assays with clonal SIVmac

SUBMITTER: Moriarty RV 

PROVIDER: S-EPMC7810186 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature