Regulation of mitochondrial dynamics in 2-methoxyestradiol-mediated osteosarcoma cell death.

ABSTRACT: Osteosarcoma (OS) is one of the most malignant tumors of childhood and adolescence. Research on mitochondrial dynamics (fusion/fission) and biogenesis has received much attention in last few years, as they are crucial for death of cancer cells. Specifically, it was shown that increased expression of the cytoplasmic dynamin-related protein 1 (Drp1) triggers mitochondrial fission (division), which activates BAX and downstream intrinsic apoptosis, effectively inhibiting OS growth. In the presented study, human OS cells (metastatic 143B OS cell line) were incubated with 2-methoxyestradiol (2-ME) at both physiologically and pharmacologically relevant concentrations. Cell viability was determined by the MTT assay. Confocal microscopy and western blot methods were applied to examine changes in Dr

SUBMITTER: Gorska-Ponikowska M 

PROVIDER: S-EPMC7811003 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature