Human neuronal signaling and communication assays to assess functional neurotoxicity.

ABSTRACT: Prediction of drug toxicity on the human nervous system still relies mainly on animal experiments. Here, we developed an alternative system allowing assessment of complex signaling in both individual human neurons and on the network level. The LUHMES cultures used for our approach can be cultured in 384-well plates with high reproducibility. We established here high-throughput quantification of free intracellular Ca²⁺ concentrations [Ca²⁺]_i as broadly applicable surrogate of neuronal activity and verified the main processes by patch clamp recordings. Initially, we characterized the expression pattern of many neuronal signaling components and selected the purinergic receptors to demonstrate the applicability of the [Ca²⁺]_i signals for q

SUBMITTER: Loser D 

PROVIDER: S-EPMC7811517 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature