Ex vivo MR microscopy of a human brain with multiple sclerosis: Visualizing individual cells in tissue using intrinsic iron.

ABSTRACT:

Purpose

To perform magnetic resonance microscopy (MRM) on human cortex and a cortical lesion as well as the adjacent normal appearing white matter. To shed light on the origins of MRI contrast by comparison with histochemical and immunostaining.

Methods

3D MRM at a nominal isotropic resolution of 15 and 18 µm was performed on 2 blocks of tissue from the brain of a 77-year-old man who had MS for 47 years. One block contained normal appearing cortical gray matter (CN block) and adjacent normal appearing white matter (NAWM), and the other also included a cortical lesion (CL block). Postmortem ex-vivo MRI was performed at 11.7T using a custom solenoid coil and T₂*-weighted 3D GRE sequence. Histochemical and immunostaining were done after paraffin embedding for iron, m

SUBMITTER: Nair G 

PROVIDER: S-EPMC7811778 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature