Comparative analysis and implications of the chloroplast genomes of three thistles (Carduus L., Asteraceae).

ABSTRACT:

Background

Carduus, commonly known as plumeless thistles, is a genus in the Asteraceae family that exhibits both medicinal value and invasive tendencies. However, the genomic data of Carduus (i.e., complete chloroplast genomes) have not been sequenced.

Methods

We sequenced and assembled the chloroplast genome (cpDNA) sequences of three Carduus species using the Illumina Miseq sequencing system and Geneious Prime. Phylogenetic relationships between Carduus and related taxa were reconstructed using Maximum Likelihood and Bayesian Inference analyses. In addition, we used a single nucleotide polymorphism (SNP) in the protein coding region of the matK gene to develop molecular markers to distinguish C. crispus from C. acanthoides a

SUBMITTER: Jung J 

PROVIDER: S-EPMC7811785 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature