Up-regulation of cofilin-1 in cell senescence associates with morphological change and p27^kip1 -mediated growth delay.

ABSTRACT: Morphological change is an explicit characteristic of cell senescence, but the underlying mechanisms remains to be addressed. Here, we demonstrated, after a survey of various actin-binding proteins, that the post-translational up-regulation of cofilin-1 was essential for the reduced rate of actin depolymerization morphological enlargement in senescent cells. Additionally, up-regulated cofilin-1 mainly existed in the serine-3 phosphorylated form, according to the 2D gel immunoblotting assay. The up-regulation of cofilin-1 was also detected in aged mammalian tissues. The over-expression of wild-type cofilin-1 and constitutively phosphorylated cofilin-1 promoted cell senescence with an increased cell size. Additionally, senescent phenotypes were also reduced by knockdown of total cofilin-1, w

SUBMITTER: Tsai CH 

PROVIDER: S-EPMC7811848 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature