Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs.

ABSTRACT: The sex-determining region on the Y chromosome (SRY) is thought to be the central genetic element of male sex development in mammals. Pathogenic modifications within the SRY gene are associated with a male-to-female sex reversal syndrome in humans and other mammalian species, including rabbits and mice. However, the underlying mechanisms are largely unknown. To understand the biological function of the SRY gene, a site-directed mutational analysis is required to investigate associated phenotypic changes at the molecular, cellular, and morphological level. Here, we successfully generated a knockout of the porcine SRY gene by microinjection of two CRISPR-Cas ribonucleoproteins, targeting the centrally located "high mobility group" (HMG), followed by a frameshift mutation of the downstream SR

SUBMITTER: Kurtz S 

PROVIDER: S-EPMC7812820 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature