Lipases of germinating jojoba seeds efficiently hydrolyze triacylglycerols and wax esters and display wax ester-synthesizing activity.

ABSTRACT:

Background

Simmondsia chinensis (jojoba) is the only plant known to store wax esters instead of triacylglycerols in its seeds. Wax esters are composed of very-long-chain monounsaturated fatty acids and fatty alcohols and constitute up to 60% of the jojoba seed weight. During jojoba germination, the first step of wax ester mobilization is catalyzed by lipases. To date, none of the jojoba lipase-encoding genes have been cloned and characterized. In this study, we monitored mobilization of storage reserves during germination of jojoba seeds and performed detailed characterization of the jojoba lipases using microsomal fractions isolated from germinating seeds.

Results

During 26 days of germination, we observed a 60-70% decrease in wax ester content in the seeds, which was accom

SUBMITTER: Kawinski A 

PROVIDER: S-EPMC7814598 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature