Development of mPing-based activation tags for crop insertional mutagenesis.

ABSTRACT: Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion

SUBMITTER: Johnson A 

PROVIDER: S-EPMC7814626 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature