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Accurate bulk quantitation of droplet digital PCR.

ABSTRACT: Droplet digital PCR provides superior accuracy in nucleic acid quantitation. The requirement of microfluidics to generate and analyze the emulsions, however, is a barrier to its adoption, particularly in low resource or clinical settings. Here, we report a novel method to prepare ddPCR droplets by vortexing and readout the results by bulk analysis of recovered amplicons. We demonstrate the approach by accurately quantitating SARS-CoV-2 sequences using entirely bulk processing and no microfluidics. Our approach for quantitating reactions should extend to all digital assays that generate amplicons, including digital PCR and LAMP conducted in droplets, microchambers, or nanoliter wells. More broadly, our approach combines important attributes of ddPCR, including enhanced accuracy and robustness to inhibition, with the high-volume sample processing ability of quantitative PCR.

SUBMITTER: Sun C

PROVIDER: S-EPMC7814815 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Accurate bulk quantitation of droplet digital PCR.

Sun Chen C Liu Leqian L Vasudevan Harish N HN Chang Kai-Chun KC Abate Adam R AR

bioRxiv : the preprint server for biology 20210115

Droplet digital PCR provides superior accuracy in nucleic acid quantitation. The requirement of microfluidics to generate and analyze the emulsions, however, is a barrier to its adoption, particularly in low resource or clinical settings. Here, we report a novel method to prepare ddPCR droplets by vortexing and readout the results by bulk analysis of recovered amplicons. We demonstrate the approach by accurately quantitating SARS-CoV-2 sequences using entirely bulk processing and no microfluidic ...[more]

PMID: 33469578

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