GLI3 Promotes Invasion and Predicts Poor Prognosis in Colorectal Cancer.

ABSTRACT:

Purpose

The epithelial-mesenchymal transition (EMT) is a key hallmark of cancer which promotes malignant progression, especially during the process of cancer invasion. A better understanding of EMT will help elucidate the molecular mechanism underlying colorectal cancer (CRC) metastasis and may provide new insights into the identification of potential biomarkers and therapeutic targets.

Methods

A series of bioinformatic approaches were combined and identify GLI3 as a potential key regulator in EMT. In vitro experiments were performed to knockdown GLI3 expression in two CRC cell lines and to reveal the oncogenic role of GLI3 in CRC. qRT-PCR and western blot were performed to show the influence of GLI3 in EMT and downstream pathways. The Kaplan-Meier analysis and log-rank test

SUBMITTER: Shen M 

PROVIDER: S-EPMC7814942 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature