Vm-milR37 contributes to pathogenicity by regulating glutathione peroxidase gene VmGP in Valsa mali.

ABSTRACT: MicroRNAs play important roles in various biological processes by regulating their corresponding target genes. However, the function and regulatory mechanism of fungal microRNA-like RNAs (milRNAs) are still largely unknown. In this study, a milRNA (Vm-milR37) was isolated and identified from Valsa mali, which causes the most serious disease on the trunk of apple trees in China. Based on the results of deep sequencing and quantitative reverse transcription PCR, Vm-milR37 was found to be expressed in the mycelium, while it was not expressed during the V. mali infection process. Overexpression of Vm-milR37 did not affect vegetative growth, but significantly decreased pathogenicity. Based on degradome sequencing, the target of Vm-milR37 was identified as VmGP, a glutathione peroxidase. The exp

SUBMITTER: Feng H 

PROVIDER: S-EPMC7814965 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature