Ontology highlight
ABSTRACT:
SUBMITTER: Zhu R
PROVIDER: S-EPMC7815195 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Zhu Rui R Huang Yifan Y Zhao Jingfu J Zhong Jieqiang J Mechref Yehia Y
Analytical chemistry 20200706 14
Protein glycosylation is involved in many biological processes and physiological functions. Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis. Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases. These findings permitted the isomeric separation ...[more]