Rheumatoid arthritis CD14⁺ monocytes display metabolic and inflammatory dysfunction, a phenotype that precedes clinical manifestation of disease.

ABSTRACT:

Introduction

This study investigates the metabolic activity of circulating monocytes and their impact on pro-inflammatory responses in RA and explores whether this phenotype is already primed for inflammation before clinical manifestations of disease.

Methods

Blood was collected and CD14⁺ monocytes isolated from healthy control donors (HC), individuals at-risk (IAR) and RA patients. Monocyte frequency in blood and synovial tissue was assessed by flow cytometry. Inflammatory responses and metabolic analysis ± specific inhibitors were quantified by RT-PCR, Western blot, migration assays, Seahorse-XFe-technology, mitotracker assays and transmission electron microscopy. Transcriptomic analysis was performed on HC, IAR and RA synovial tissue.

Results

CD14^+<

SUBMITTER: McGarry T 

PROVIDER: S-EPMC7815439 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature