[¹⁸F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts.

ABSTRACT: Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft survival. We studied [¹⁸F]-sodium fluoride ([¹⁸F]-NaF) imaging of microcalcifications in donor kidneys (n = 7) and explanted kidney allografts (n = 13). Three µm paraffin-embedded serial sections were used for histological evaluation of calcification (Alizarin Red; Von Kossa staining) and ex-vivo [¹⁸F]-NaF autoradiography. The images were fused to evaluate if microcalcification areas corresponded with [¹⁸F]-NaF uptake areas. Based on histological analyses, tubulointerstitial and glomerular microcalcifications were present in 19/20 and 7/20 samples, respectively. Using autoradiography

SUBMITTER: Benjamens S 

PROVIDER: S-EPMC7815841 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature