Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies.

ABSTRACT: Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins. Protein A purification is specific and efficient. However, there still remain several issues to be addressed, such as incomplete clearance of impurities including host cell proteins, DNA, aggregates, etc. In addition, the effects of wash buffers in protein A purification on the physicochemical characteristics of antibodies have yet to be fully understood. Here we found a new purification protocol for monoclonal antibodies that can improve physicochemical properties of monoclonal antibodies simply by inserting an additional wash step with a basic buffer after the capture step to the conventional protein A purification. Th

SUBMITTER: Imura Y 

PROVIDER: S-EPMC7815873 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature