Efficient Production of Pyruvate Using Metabolically Engineered Lactococcus lactis.

ABSTRACT: Microbial production of commodity chemicals has gained increasing attention and most of the focus has been on reducing the production cost. Selecting a suitable microorganism, which can grow rapidly on cheap feedstocks, is of key importance when developing an economically feasible bioprocess. We chose Lactococcus lactis, a well-characterized lactic acid bacterium, as our microbial host to produce pyruvate, which is a commodity chemical with various important applications. Here we report the engineering of Lactococcus lactis into becoming an efficient microbial platform for producing pyruvate. The strain obtained, FS1076 (MG1363 Δ³ ldh Δpta ΔadhE Δals), was able to produce pyruvate as the sole product. Since all the competitive pathways h

SUBMITTER: Suo F 

PROVIDER: S-EPMC7815928 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature