A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.

ABSTRACT:

Introduction

Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings.

Methods

All experiments were performed on a mobile UV/VIS spectrophotometer. The levofloxacin concentration was quantified by using the amplitude of the second-order spectrum between 300 and 400 nm of seven calibrators. The concentration of spiked samples was calculated from the spectrum amplitude using linear regression. The method was validated for se

SUBMITTER: Alffenaar JC 

PROVIDER: S-EPMC7816168 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature