Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.

ABSTRACT:

Background

Eukaryotes use distinct networks of biogenesis factors to synthesize, fold, monitor, traffic, and secrete proteins. During heterologous expression, saturation of any of these networks may bottleneck titer and yield. To understand the flux through various routes into the early secretory pathway, we quantified the global and membrane-associated translatomes of Komagataella phaffii.

Results

By coupling Ribo-seq with long-read mRNA sequencing, we generated a new annotation of protein-encoding genes. By using Ribo-seq with subcellular fractionation, we quantified demands on co- and posttranslational translocation pathways. During exponential growth in rich media, protein components of the cell-wall represent the greatest number of nascent chains entering the ER. Transc

SUBMITTER: Alva TR 

PROVIDER: S-EPMC7816318 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature