The diagnostic value of lower glucose consumption for IDH1 mutated gliomas on FDG-PET.

ABSTRACT:

Background

Non-invasive diagnosis of IDH1 mutation for gliomas has great clinical significance, and PET has natural advantage to detect metabolism, as IDH mutated gliomas share lower glucose consumption.

Methods

Clinical data of patients with gliomas and ¹⁸F-FDG PET were retrospectively reviewed. Receiver operating characteristic curve (ROC) analysis was conducted, and standard uptake value (SUV) was estimated in combination with grades or IDH1 mutation. The glucose consumption was investigated with U251 cells expressing wild-type or mutated IDH1 by glucose assay. Quantification of glucose was determined by HPLC in clinical tissues. Meanwhile, bioinformatics and western blot were applied to analyze the expression level of metabolic enzymes (e.g. HK1, PKM2, PC) in

SUBMITTER: Liu FM 

PROVIDER: S-EPMC7816361 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature