Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.

ABSTRACT:

Background

Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depending on fossil resources. Therefore, we engineered an Enterobacter cloacae budC and ldhA double-deletion strain (namely, EC∆budC∆ldhA) to redirect carbon fluxes and optimized the culture conditions to co-produce succinic acid and acetoin.

Results

In this work, E. cloacae was metabolically engineered to enhance its combined succinic acid and acetoin production during fermentation. Strain EC∆budC∆ldhA was constructed by deleting 2,3-butanediol dehydrogenase (bud

SUBMITTER: Su HY 

PROVIDER: S-EPMC7816431 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature