Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro.

ABSTRACT:

Background

Toxicological evaluation of engineered nanomaterials (ENMs) is essential for occupational health and safety, particularly where bulk manufactured ENMs such as few-layer graphene (FLG) are concerned. Additionally, there is a necessity to develop advanced in vitro models when testing ENMs to provide a physiologically relevant alternative to invasive animal experimentation. The aim of this study was to determine the genotoxicity of non-functionalised (neutral), amine- and carboxyl-functionalised FLG upon both human-transformed type-I (TT1) alveolar epithelial cell monocultures, as well as co-cultures of TT1 and differentiated THP-1 monocytes (d.THP-1 (macrophages)).

Results

In monocultures, TT1 and d.THP-1 macrophages showed a statistically significant (p < 0.05) cyt

SUBMITTER: Burgum MJ 

PROVIDER: S-EPMC7816456 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature