Variable blood processing procedures contribute to plasma proteomic variability.

ABSTRACT:

Background

Plasma is a potentially rich source of protein biomarkers for disease progression and drug response. Large multi-center studies are often carried out to increase the number of samples analyzed in a given study. This may increase the chances of variation in blood processing and handling, leading to altered proteomic results. This study evaluates the impact of blood processing variation on LC-MS/MS proteomic analysis of plasma.

Methods

Initially two batches of patient plasma samples (120 and 204 samples, respectively) were analyzed using LC-MS/MS shotgun proteomics. Follow-up experiments were designed and carried out on healthy donor blood in order to examine the effects of different centrifugation conditions, length of delay until first centrifugation, storage temp

SUBMITTER: Halvey P 

PROVIDER: S-EPMC7816468 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature