Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates.

ABSTRACT: Extracellular electron transfer (EET) pathways, such as those in the bacterium Shewanella oneidensis, interface cellular metabolism with a variety of redox-driven applications. However, designer control over EET flux in S. oneidensis has proven challenging because a functional understanding of its EET pathway proteins and their effect on engineering parametrizations (e.g., response curves, dynamic range) is generally lacking. To address this, we systematically altered transcription and translation of single genes encoding parts of the primary EET pathway of S. oneidensis, CymA/MtrCAB, and examined how expression differences affected model-fitted parameters for Fe(III) reduction kinetics. Using a suite of plasmid-based inducible circuits maintained by appropriate S.

SUBMITTER: Dundas CM 

PROVIDER: S-EPMC7816516 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature