Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.

ABSTRACT: Use of telomere length (TL) as a biomarker for various environmental exposures and diseases has increased in recent years. Various methods have been developed to measure telomere length. Polymerase chain reaction (PCR)-based methods remain wide-spread for population-based studies due to the high-throughput capability. While several studies have evaluated the repeatability and reproducibility of different TL measurement methods, the results have been variable. We conducted a literature review of TL measurement cross-method comparison studies that included a PCR-based method published between January 1, 2002 and May 25, 2020. A total of 25 articles were found that matched the inclusion criteria. Papers were reviewed for quality of methodologic reporting of sample and DNA quality, PCR assay c

SUBMITTER: Lindrose AR 

PROVIDER: S-EPMC7817045 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature