Dataset Information

Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis.

ABSTRACT: Macrophages are the principal immune cells of the epididymis and testis, but their origins, heterogeneity, development, and maintenance are not well understood. Here, we describe distinct populations of epididymal and testicular macrophages that display an organ-specific cellular identity. Combining in vivo fate-mapping, chimeric and parabiotic mouse models with in-depth cellular analyses, we found that CD64hiMHCIIlo and CD64loMHCIIhi macrophage populations of epididymis and testis arise sequentially from yolk sac erythro-myeloid progenitors, embryonic hematopoiesis, and nascent neonatal monocytes. While monocytes were the major developmental source of both epididymal and testicular macrophages, both populations self-maintain in the steady-state independent of bone marrow hematopoietic precursors. However, after radiation-induced macrophage ablation or during infection, bone marrow-derived circulating monocytes are recruited to the epididymis and testis, giving rise to inflammatory macrophages that promote tissue damage. These results define the layered ontogeny, maintenance and inflammatory response of macrophage populations in the male reproductive organs.

SUBMITTER: Wang M

PROVIDER: S-EPMC7817195 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis.

Wang Ming M Yang Yalong Y Cansever Dilay D Wang Yiming Y Kantores Crystal C Messiaen Sébastien S Moison Delphine D Livera Gabriel G Chakarov Svetoslav S Weinberger Tobias T Stremmel Christopher C Fijak Monika M Klein Britta B Pleuger Christiane C Lian Zhexiong Z Ma Wentao W Liu Qingzhi Q Klee Kathrin K Händler Kristian K Ulas Thomas T Schlitzer Andreas A Schultze Joachim L JL Becher Burkhard B Greter Melanie M Liu Zhaoyuan Z Ginhoux Florent F Epelman Slava S Schulz Christian C Meinhardt Andreas A Bhushan Sudhanshu S

Proceedings of the National Academy of Sciences of the United States of America 20210101 1

Macrophages are the principal immune cells of the epididymis and testis, but their origins, heterogeneity, development, and maintenance are not well understood. Here, we describe distinct populations of epididymal and testicular macrophages that display an organ-specific cellular identity. Combining in vivo fate-mapping, chimeric and parabiotic mouse models with in-depth cellular analyses, we found that CD64hiMHCIIlo and CD64loMHCIIhi macrophage popula ...[more]

PMID: 33372158

Similar Datasets

Project description:Linuron is well known for its antiandrogenic property. However, the effects of linuron on testicular and epididymal pro- and antioxidant status are not well defined. On the other hand, α-lipoic acid is well known as universal antioxidant. Therefore, the purpose of this study was twofold: firstly to investigate whether linuron exposure alters antioxidant status in the testis and epididymis of rats and if so, whether the supplementation of α-lipoic acid mitigates linuron-induced oxidative toxicity in rats. To address this question, α-lipoic acid at a dose of 70 mg/Kg body weight (three times a week) was administered to linuron exposed rats (10 or 50 mg/Kg body weight, every alternate day over a period of 60 days), and the selected reproductive endpoints were analyzed after 60 days. Respective controls were maintained in parallel. Linuron at selected doses reduced testicular daily sperm count, and epididymal sperm count, sperm motility, sperm viability, and number of tail coiled sperm, reduced activity levels of 3β- and 17β-hydroxysteroid dehydrogenases, decreased expression levels of StAR mRNA, inhibition of testosterone levels, and elevated levels of testicular cholesterol in rats over controls. Linuron intoxication deteriorated the structural integrity of testis and epididymis associated with reduced the reproductive performance over controls. Conversely, α-lipoic acid supplementation enhanced sperm quality and improved the testosterone synthesis pathway in linuron exposed rats over its respective control. Administration of α-lipoic acid restored inhibition of testicular and epididymal enzymatic (superoxide dismutase, catalase, glutathione reductase, glutathione peroxidise) and non-enzymatic (glutathione content), increased lipid peroxidation and protein carbonyl content produced by linuron in rats. α-lipoic acid supplementation inhibited the expression levels of testicular caspase-3 mRNA levels and also its activity in linuron treated rats. To summate, α-lipoic acid-induced protection of reproductive health in linuron treated rats could be attributed to its antioxidant, and steroidogenic properties.

Dataset Information

Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis.

Publications

Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets