Mfd regulates RNA polymerase association with hard-to-transcribe regions in vivo, especially those with structured RNAs.

ABSTRACT: RNA polymerase (RNAP) encounters various roadblocks during transcription. These obstacles can impede RNAP movement and influence transcription, ultimately necessitating the activity of RNAP-associated factors. One such factor is the bacterial protein Mfd, a highly conserved DNA translocase and evolvability factor that interacts with RNAP. Although Mfd is thought to function primarily in the repair of DNA lesions that stall RNAP, increasing evidence suggests that it may also be important for transcription regulation. However, this is yet to be fully characterized. To shed light on Mfd's in vivo functions, we identified the chromosomal regions where it associates. We analyzed Mfd's impact on RNAP association and transcription regulation genome-wide. We found that Mfd represses RNAP associati

SUBMITTER: Ragheb MN 

PROVIDER: S-EPMC7817204 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature