E3 ubiquitin ligase Mindbomb 1 facilitates nuclear delivery of adenovirus genomes.

ABSTRACT: The journey from plasma membrane to nuclear pore is a critical step in the lifecycle of DNA viruses, many of which must successfully deposit their genomes into the nucleus for replication. Viral capsids navigate this vast distance through the coordinated hijacking of a number of cellular host factors, many of which remain unknown. We performed a gene-trap screen in haploid cells to identify host factors for adenovirus (AdV), a DNA virus that can cause severe respiratory illness in immune-compromised individuals. This work identified Mindbomb 1 (MIB1), an E3 ubiquitin ligase involved in neurodevelopment, as critical for AdV infectivity. In the absence of MIB1, we observed that viral capsids successfully traffic to the proximity of the nucleus but ultimately fail to deposit their genomes wit

SUBMITTER: Sarbanes SL 

PROVIDER: S-EPMC7817214 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature