STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing.

ABSTRACT: The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.

SUBMITTER: Aoki K 

PROVIDER: S-EPMC7817837 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature