Bronchiolitis and recurrent wheezing are distinguished by type 2 innate lymphoid cells and immune response.

ABSTRACT:

Background

Recurrent wheezing (RW) is frequently developed in infants that have suffered bronchiolitis (BCH) during first months of life, but the immune mechanism underlying is not clear. The goal was to analyze the innate immune response that characterizes BCH and RW.

Methods

Ninety-eight and seventy hospitalized infants with BCH or RW diagnosis, respectively, were included. Nasopharyngeal aspirate (NPA) was processed. Cellular pellet was employed to evaluate type 2 innate lymphoid cells (ILC2) by flow cytometry and mRNA expression assays by semi-quantitative real-time PCR (qRT-PCR). In supernatant, twenty-seven pro-inflammatory and immunomodulatory factors, as well as lipid mediators and nitrites, were evaluated by ELISA and Luminex.

Results

Bronchiolitis patients s

SUBMITTER: Sastre B 

PROVIDER: S-EPMC7818223 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature