A new clustering method identifies multiple sclerosis-specific T-cell receptors.

ABSTRACT:

Objective

To characterize T-cell receptors (TCRs) and identify target epitopes in multiple sclerosis (MS).

Methods

Peripheral blood mononuclear cells were obtained from 39 MS patients and 19 healthy controls (HCs). TCR repertoires for α/β/δ/γ chains, TCR diversity, and V/J usage were determined by next-generation sequencing. TCR β chain repertoires were compared with affectation status using a novel clustering method, Grouping of Lymphocyte Interactions by Paratope Hotspots (GLIPH). Cytomegalovirus (CMV)-IgG was measured in an additional 113 MS patients and 93 HCs. Regulatory T cells (Tregs) were measured by flow cytometry.

Results

TCR diversity for all four chains decreased with age. TCRα and TCRβ diversity was higher in MS patients (P = 0.0015 and 0.024, respectivel

SUBMITTER: Hayashi F 

PROVIDER: S-EPMC7818280 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature